Millipore Amicon Ultra 0.5 mL Centrifugal Filters, 3 kDa Ultracel - Instruction Manual

1. Uvod

The Millipore Amicon Ultra 0.5 mL Centrifugal Filters are designed for rapid concentration and purification of biological samples. Featuring a low-binding Ultracel-YM regenerated cellulose membrane with a 3 kDa molecular weight cut-off, these filters are ideal for dilute protein solutions (ng/mL to µg/mL range), antibodies, and nucleic acids. They are compatible with standard 1.5 mL tube fixed-angle rotors.

Tipične aplikacije vključujejo:

• Concentrating and purifying proteins and antibodies.
• Concentrating and purifying nucleic acids (as an alternative to ethanol precipitation).
• Desalting and buffer exchange.
• Removal of primers, linkers, and unincorporated labels.

2. Značilnosti izdelka

• Visoka Sample Recovery: Achieves solute recoveries typically greater than 95%.
• Unique Vertical Housing: Designed for efficient filtration.
• Vsestranska združljivost: Suitable for use in standard 1.5 mL tube fixed-angle rotors.
• Širok obseg uporabe: Effective for concentrating and purifying proteins, antibodies, and nucleic acids, as well as for desalting, buffer exchange, and removal of small molecules.

3. Nastavitev in priprava

Preden začnete, se prepričajte, da imate naslednje:

• Amicon Ultra 0.5 mL Centrifugal Filters.
• Centrifuge with a fixed-angle rotor capable of accommodating 1.5 mL tubes and reaching up to 14,000 x g.
• Sample rešitev.
• Pipete in konice.

Priprava:

1. Inspect each filter unit for any damage. Do not use damaged units.
2. Ensure your centrifuge rotor is clean and balanced.

Image 1: A Millipore Amicon Ultra 0.5 mL Centrifugal Filter unit. This image shows the compact design of the filter, consisting of a sample reservoir and a collection tube, ready for use in a centrifuge.

4. Navodila za uporabo

Za optimalno delovanje sledite tem korakom:

1. Dodajte Sample: Pipette up to 500 µL of your sample solution into the upper reservoir of the Amicon Ultra filter unit. Ensure the sample does not exceed the maximum volume mark.
2. Centrifugiranje: Insert the assembled filter unit into a fixed-angle rotor. Ensure the rotor is properly balanced. Centrifuge at the recommended speed and time.
   • For most protein applications, centrifuge at up to 14,000 x g.
   • For DNA/RNA applications, centrifuge at 500 x g.
   • Centrifugation time will vary depending on the desired final concentrate volume, sample viscosity, and temperature. Refer to typical performance data for guidance.
3. Sample Recovery: After centrifugation, remove the filter unit from the rotor.
   • To recover the concentrated sample, invert the filter unit into a clean collection tube.
   • Centrifuge again at 1,000 x g for 2 minutes to transfer the concentrated sample from the filter device into the collection tube.
   • The typical final concentrate volume is 5 to 15 µL.
4. Desalting/Buffer Exchange (Optional): For desalting or buffer exchange, after the initial concentration step, add an appropriate volume of the new buffer to the concentrated sample in the filter unit and centrifuge again. Repeat this process as needed.

5. Vzdrževanje in shranjevanje

Amicon Ultra Centrifugal Filters are designed for single use only. Do not attempt to clean or reuse the filters, as this may compromise performance and introduce contamination.

• Shranjevanje: Store unused filters in their original packaging at room temperature (15-30°C) in a dry place, away from direct sunlight.
• Odstranjevanje: Dispose of used filter units and any biological waste according to local regulations and laboratory safety protocols.

6. Odpravljanje težav

Če med delovanjem naletite na težave, upoštevajte naslednje:

• Nizka Sample Recovery:
  • Ensure the final inversion spin is performed correctly to recover all concentrated sample.
  • Verify that the membrane was not damaged during handling.
  • Check for protein aggregation or precipitation on the membrane.
• Slow Filtration Rate:
  • Visoka sample viscosity can slow filtration. Consider diluting the sample if appropriate.
  • Particulate matter in the sample can clog the membrane. Pre-filter samples, če je potrebno.
  • Ensure the centrifuge speed is set correctly.
• Sample Leakage:
  • Ensure the filter unit is properly assembled and seated in the centrifuge rotor.
  • Do not exceed the maximum sampvolumen.

7. Specifikacije

8. Garancija in podpora

For product support, technical assistance, or warranty information, please contact Millipore customer service or your authorized distributor. Refer to the official Millipore website for the most current contact details and product documentation.